Owaisi was born in Hyderabad on 14 June 1970. He studied in the Hyderabad Public School till tenth grade and completed his intermediate from St. Mary's Junior College in Hyderabad. Owaisi studied medicine for two years in Gulbarga but quit during his second year of MBBS course to enter politics. In 1995, Owaisi married a Christian woman(Sabina), who converted to Islam and took the name of Sabina Farzana. The marriage was opposed by his father Salahuddin Owaisi who refused to forgive Akbaruddin. Then Akbaruddin Owaisi and his father reconciled in 1998.The couple has one daughter Fathima and son Nooruddin.
Akbaruddin Owaisi is the managing director of Owaisi hospital, which was set up by his late father Sultan Salahuddin Owaisi for the welfare of the Muslims.